RESUMO
The annual dynamics of whole mature almond tree nutrient remobilization in spring and the accumulation of nutrients in perennial tissues during the year were determined by sequential coring, tissue sampling, nutrient analysis, whole tree excavation and biomass estimation for trees grown under four nitrogen rate treatments 140 kg ha-1 N (N140), 224 kg ha-1 N (N224), 309 kg ha-1 N (N309), and 392 kg ha-1 N (N392) over 2 years. Whole tree perennial organ N content was greatest in dormancy then declined through bud swell, flowering and fruit set, achieving the lowest total whole tree nutrient content of perennial organs by March 12 [12-14 days after full bloom (DAFB)] coincident with 60-70% leaf expansion. During this period no net increment in whole tree N content (annual plus perennial N) was observed indicating that tree demand for N for bud break, flowering, fruit set and leaf out was met by remobilized stored N and that there was no net N uptake from soil. Remobilizable N increased with increasing N application up to N309 and was maximal at 44.4 ± 4 kg ha-1 and 37.5 ± 5.7 kg ha-1 for the optimally fertilized N309 in 2012 and 2013 respectively. Net increases in perennial organ N (stored N) commenced 41 DAFB and continued through full leaf abscission at 249 DAFB. Total annual N increment in perennial organs varied from 25 to 60 kg ha-1 and was strongly influenced by N rate and tree yield. N remobilized from senescing leaves contributed from 11 to 15.5 ± 0.6 kg ha-1 to perennial stored N. Similar patterns of nutrient remobilization and storage were observed for P, K, and S with maximal whole tree perennial storage occurring during dormancy and remobilization of that stored P, K, S to support annual tree demands through to fruit set and 70-100% leaf development. Net annual increment in perennial organ P, K, S commenced 98 DAFB and continued through full leaf abscission at 249 DAFB. Organ specific contribution to remobilizable and stored nutrients changes over the growing season are presented. Details of the pattern of perennial organ nutrient allocation, storage, and remobilization provides a framework for the optimal management of nutrients in almond with relevance for other deciduous tree species.
RESUMO
Nitrogen (N) fertilizer use has simultaneously increased global food production and N losses, resulting in degradation of water quality and climate pollution. A better understanding of N application rates and crop and environmental response is needed to optimize management of agroecosystems. Here we show an orchard agroecosystem with high N use efficiency promoted substantial gains in carbon (C) storage, thereby lowering net global warming potential (GWP). We conducted a 5-year whole-system analysis comparing reduced (224 kg N ha-1 yr-1) and intensive (309 kg N ha-1 yr-1) fertilizer N rates in a California almond orchard. The intensive rate increased net primary productivity (Mg C ha-1) and significantly increased N productivity (kg N ha-1) and net N mineralization (mg N kg-1 soil d-1). Use of 15N tracers demonstrated short and long-term mechanisms of soil N retention. These low organic matter soils (0.3-0.5%) rapidly immobilized fertilizer nitrate within 36 h of N application and 15N in tree biomass recycled back into soil organic matter over five years. Both fertilizer rates resulted in high crop and total N recovery efficiencies of 90% and 98% for the reduced rate, and 72% and 80% for the intensive rate. However, there was no difference in the proportion of N losses to N inputs due to a significant gain in soil total N (TN) in the intensive rate. Higher soil TN significantly increased net N mineralization and a larger gain in soil organic carbon (SOC) from the intensive rate offset nitrous oxide (N2O) emissions, leading to significantly lower net GWP of -1.64 Mg CO2-eq ha-1 yr-1 compared to -1.22 Mg CO2-eq ha-1 yr-1 for the reduced rate. Our study demonstrates increased N cycling and climate mitigation from intensive fertilizer N use in this orchard agroecosystem, implying a fundamentally different result than seen in conventional annual cropping systems.
RESUMO
Working in tandem with root exclusion, stems may provide salt-tolerant woody perennials with some additional capacity to restrict sodium (Na) and chloride (Cl) accumulation in leaves. The Pistacia genus, falling at the nexus of salt tolerance and human intervention, provided an ideal set of organisms for studying the influences of both variable root exclusion and potentially variable discontinuities at the bud union on stem processes. In three experiments covering a wide range of salt concentrations (0 to 150 mM NaCl) and tree ages (1, 2 and 10 years) as well as nine rootstock-scion combinations we show that proportional exclusion of both Na and Cl reached up to ~85% efficacy, but efficacy varied by both rootstock and budding treatment. Effective Na exclusion was augmented by significant retrieval of Na from the xylem sap, as evidenced by declines in the Na concentrations of both sap and wood tissue along the transpiration stream. However, while we observed little to no differences between the concentrations of the two ions in leaves, analogous declines in sap concentrations of Cl were not observed. We conclude that some parallel but separate mechanism must be acting on Cl to provide leaf protection from toxicity specific to this ion and suggest that this mechanism is recirculation of Cl in the phloem. The presented findings underline the importance of holistic assessments of salt tolerance in woody perennials. In particular, greater emphasis might be placed on the dynamics of salt sequestration in the significant storage volumes offered by the stems of woody perennials and on the potential for phloem discontinuity introduced with a bud/graft union.
